Effects of aging and LPS on inflammatory cytokines and P2X7receptor mRNA expression in brains of mice

摘要：Objective:To explore the effect of tumor necrosis factor-α (TNF-α),interleukin (IL)-6,IL-18,IL-1β,and aging on the expression of P2X7 receptor (P2X7R) rnRNA in the brains of mice with lipopolysaccharide (LPS) treatment.Methods:Young (4 months old) and aged (18 months old) male C57BL/6 mice were randomly divided into 5 groups (n =7).All of the mice were injected intraperitoneally with LPS (0.5 mg/kg).After 0,3,24,48,and 72 h,the mRNA expressions of TNF-α,IL-6,IL-18,IL-1β,and P2X7R in mice's brains were determined by qPCR.Results:In brains of young mice,the highest expressions of IL-6,IL-18 and IL-1β were found at 3 h,but for TNF-α and P2X7R,the highest expressions were found at 24 h.For the aged mice,the expressions of TNF-α,IL-6,IL-1β,and P2X7R reached the highest level at 3 h,but IL-18 reached the highest level at 24 h.Aged mice showed significantly higher level of TNF-α at 0,3,48,and 72 h than young mice.The similar results were observed for P2X7R at 0,3 and 72 h in aged mice.Conclusion:The mRNA expression of inflammatory cytokines and P2X7R in the brains of mice were consistent with the same time points after LPS treatment.Furthermore,they were higher in aged mice compared with young mice.This result suggested that P2X7R may be involved in the development of brain aging and relevant diseases of mice by regulating the expression of inflammatory cytokines.