期刊简介
本刊是由广西医科大学主办的国内外公开发行的医学学术期刊。内容丰富，形式多样，科学性强，有实用性。主要设有论著、经验交流、调查研究、技术与方法、护理园地、病例报告等栏目。
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首页>广西医科大学学报杂志
- 杂志名称：广西医科大学学报杂志
- 主管单位：广西医科大学
- 主办单位：广西医科大学
- 国际刊号：1005-930X
- 国内刊号：45-1211/R
- 出版周期：双月刊
期刊荣誉：全国高校自然科学优秀学报期刊收录：知网收录(中), 上海图书馆馆藏, 国家图书馆馆藏, 维普收录(中), 统计源核心期刊(中国科技论文核心期刊), 万方收录(中)
Effects of miR-21 antisense oligonucleotides on proliferation,migration and autophagy of human umbilical vein endothelial cells
Lyu Dongning；Luo Xuelan；Yang Ruixia；Wang Guangyao；Zhou Dong；Gan Na；Ou Hesheng
关键词：As-miR-21, Human umbilical vein endothelial cells, Cell proliferation, Autophagy
摘要：Objective:To investigate the effects of microRNA-21 antisense nucleotide (AS-miR-21 )on the proliferation, migration and autophagy of human umbilical vein endothelial cells (HUVECs ).Methods:HUVECs were treated with 1,000 nmol/L rapamycin for 6 h (rapamycin group)or AS-miR-21 transfection followed by 1,000 nmol/L rapamycin for 6 h (AS-miR-21 + rapamycin group).HUVECs without any treatment were defined as control group.The proliferation and migration abilities of HUVECs were detected by methyl thiazolyl tetrazolium (MTT)assay,scratch wound healing assay and transwell test,respectively.The expressions of mi-crotubule-associated protein light chain 3 Ⅱ/Ⅰ (LC3 Ⅱ/Ⅰ) and Becline-1 were determined by western blotting.Results:The rapamycin group showed decreased OD value and migra-tion rate,an increased ratio of LC3 Ⅱ/Ⅰ and up-regulated expression of Beclin-1 compared with the control group (P <0.05).The AS-miR-21+rapamycin group demonstrated low-er OD value,migration rate,the number of migrated cells, and significantly higher ratio of LC3 Ⅱ/Ⅰ and Beclin-1 pro-tein expression level than the control group and the rapamy-cin group (P <0.05).Conclusion:AS-miR-21 suppressed the autophagy,proliferation and migration in the HUVECs mod-el of autophagy induced by rapamycin.
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