期刊简介
本刊是由广西医科大学主办的国内外公开发行的医学学术期刊。内容丰富，形式多样，科学性强，有实用性。主要设有论著、经验交流、调查研究、技术与方法、护理园地、病例报告等栏目。
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首页>广西医科大学学报杂志
- 杂志名称：广西医科大学学报杂志
- 主管单位：广西医科大学
- 主办单位：广西医科大学
- 国际刊号：1005-930X
- 国内刊号：45-1211/R
- 出版周期：双月刊
期刊荣誉：全国高校自然科学优秀学报期刊收录：知网收录(中), 上海图书馆馆藏, 国家图书馆馆藏, 维普收录(中), 统计源核心期刊(中国科技论文核心期刊), 万方收录(中)
Effects and mechanism of miRNA-24on the proliferation,migration and tube formation of vascular endothelial cells
Xiaojing Tao；Feng Shen；Peng Yang；Xuelan Luo；Dan Li；Yuanyuan Yan；Yongxiong Zhong；Hesheng Ou
关键词：miRNA-24, vascular endothelial growth factor, Sp1, endothelial cells, tube formation
摘要：Objective:To investigate the effect and mechanism of miRNA-24 on the proliferation,migration and tube forma tion of vascular endothelial cells.Methods:Human umbilical vein endothelial cells (HUVECs) were assigned into control,microRNA (miR) 24 overexpression and anti-miR-24 groups.The proliferation and migration abilities of HUVECs were detected by methyl thiazolyl tetrazolium (MTT) assay and scratch wound healing assay,respectively.The ability of HUVECs to form tubular structures was evaluated by a tube formation assay.The mRNA and protein expressions of vascular endothelial growth factor (VEGF) and transcription factor Sp1 were determined by RT-PCR,immunocytochemis try and western blotting,respectively.Results:The miR-24 overexpression group exhibited decreased cell proliferation and migration,and expressions of VEGF and Sp1 compared with the control group (P ＜ 0.01).No tube like network structure was formed in the miR-24 overexpression group.However,inhibition of miR 24 in HUVECs markedly in creased cell proliferation and migration,enhanced tube for mation and expressions of VEGF and Sp1 (P＜0.05 or P＜ 0.01).Conclusion:MiR-24 suppressed the proliferation,migration and tube formation of HUVECs,and the mechanism might be related to the down-regulation of VEGF expression.Sp1 might participate in this regulation process.
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