Effect and mechanism of Polygonatum sibiricum polysaccharide on osteogenic differentiation of bone mesenchymal stem cells(BMSCs)

摘要：Objective:To investigate the effects and mechanisms of Polygonatum sibiricum polysaccharide(PSP) on osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs).Methods:The mouse BMSCs were cultured and induced in osteoblast medium(OBM) containing finalconcentrations(0 mg/L,12.5 mg/L,25 mg/L,and 50 mg/L) of PSP.The proliferation and cytotoxicity of BMSCs were detected by MTT assay.Alkaline phosphatase(ALP) and Alizarin red S staining were performed after 7 days' ossification-inducing culture.The mRNA expressions of ALP,Runx2 and osteocalcin(OCN) were determined by fluorescence quantitative PCR(qPCR).The mRNA and protein expressions of Tafazzin (TAZ) (a key effector of Hippo pathway) were measured by qPCR and western blotting,respectively.Results:PSP was non-cytotoxicwithin the dose range of 12.5-50 mg/L and had no impact on the proliferation of BMSCs.The activity of ALP,the intensity of ALP staining,and the formation of mineralized nodules were increased by PSP treatment(25 and 50 mg/L) (P＜0.01).Moreover,administration of 25 mg/L PSP significantly enhanced the mRNA levels of osteoblastic differentiation makers ALP,Runx2 and OCN as well as the mRNA and protein expressions of TAZ (P ＜ 0.01).Conclusion:PSP could promote osteogenic differentiation of BMSCs,and the mechanisms might be related to the activation of TAZ in the Hippo signaling pathway.